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Chick Embryo Extract (CEE)

Name: Chick Embryo Extract (CEE) | Frozen & Lyophilised | UK
Brand: Life Science Group
Availability: InStock

Price range: £34.73 through £601.91

UK-origin chick embryo extract for neural crest stem cell and muscle cell culture. Frozen or lyophilised, 0.2 µm filtered and growth-promotion tested on every batch, with a 5-year shelf life.

Lyophilisation

Gamma Irradiated

Size

Clear

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SKU N/A Categories Animal Products, Chick Embryo Extract (CEE) Tag LSG new template preview Brand: Life Science Group

Overview

Chick Embryo Extract (CEE) is a cell culture supplement that provides essential growth factors. It is widely used to culture neural crest stem cells and muscle cells. LSG’s CEE is UK-origin and quality assured. It is available as a frozen liquid or lyophilised, so you can choose the format that suits your workflow.

Applications

Neural crest stem cells: CEE is a key supplement in neural crest stem cell medium for rat neural crest stem cells and other neural explants.
Muscle cell culture: CEE is used in myogenesis studies and to culture skeletal muscle cells.
Stem cell expansion: CEE has been used to expand many specific stem cell types.
Epigenetics research: CEE has been shown to facilitate DNA demethylation.

The product information sheet cites 26 published studies.

Production and quality

CEE is made from Bovans Goldline chick embryos. The eggs come from registered UK flocks free of clinical signs of notifiable disease. The extract is filtered to 0.2 µm and aseptically filled. Antibiotics are typically added: penicillin, streptomycin and amphotericin.

Every batch is tested negative for bacteria, yeast and fungi. Each batch is also tested by assaying the growth and differentiation of mouse muscle cells derived from the H-2Kᵇ-tsA58 transgenic mouse. Results are reported on the Certificate of Analysis.

Chick Embryo Extract in published research

Researchers have used LSG Chick Embryo Extract in published muscle and stem cell studies, including:

Nature Communications (2026) – Sarcopenia promotes tumorigenesis by disrupting NOTCH-SDC2-regulated biogenesis of muscle-derived extracellular vesicles. Goh KY et al. Nat Commun 17:5750.
0.5% CEE in the culture medium for mouse myofibres. Catalogue code cited: MD004A-UK.
Nature Communications (2026) – Impaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imaging. Sarde L et al. Nat Commun 17:1769.
0.5% CEE in muscle stem cell differentiation medium. Catalogue code cited: MD-004D-UK.
American Journal of Pathology (2025) – Pharmacologic Inhibition of Glutamate Dehydrogenase 1 Improves Functional Recovery of Neuromuscular Junctions and Muscle Function in Duchenne Muscular Dystrophy. Pereira-Nunes A et al. Am J Pathol 195(8):1537–1552.
1% CEE in satellite cell culture. Catalogue code cited: MDL-004E-UK.
Autophagy (2024) – FOXO-regulated DEAF1 controls muscle regeneration through autophagy. Goh KY et al. Autophagy.
1% CEE in muscle stem cell growth medium. Catalogue code cited: MD004A-UK.

These publications report researchers’ use of LSG products in their methods. Some name LSG as Life Science Production. Inclusion does not imply endorsement by the authors, and results depend on each study’s conditions.

Request a CEE sample: email sales@lifesciencegroup.co.uk or call +44 1234 889180.

Specifications

Origin	UK. Bovans Goldline chick embryos from registered UK flocks
Formats	Frozen liquid (aseptically filled) or lyophilised
Filtration	0.2 µm, aseptically filled
Antibiotics	Penicillin, streptomycin and amphotericin typically added; antibiotic/antimycotic-free available
Sterility	Every batch tested negative for bacteria, yeast and fungi
Growth promotion	Growth and differentiation assay on mouse muscle cells derived from the H-2Kᵇ-tsA58 transgenic mouse
Shelf life	5 years from manufacture
Storage	Frozen: -20 °C or below. Lyophilised: +4 °C
Shipping	Frozen on dry ice; lyophilised on blue ice

Ordering

Product code	Description	Pack size
MD-004A-UK	CEE, aseptically filled (frozen)	500 mL
MD-004D-UK	CEE, aseptically filled (frozen)	20 mL
MD-004E-UK	CEE, aseptically filled (frozen)	10 mL
MDL-004E-UK	CEE, lyophilised	10 mL
MDL-004E-GI-UK	CEE, lyophilised, gamma irradiated	10 mL

CEE is also available as an ultrafiltrate or without antibiotics, and in custom sizes.

FAQs

Should I choose frozen or lyophilised CEE?

Both formats are available. Frozen CEE is aseptically filled and stored at -20 °C or below. Lyophilised CEE can be stored at +4 °C, which suits labs without freezer space or that need easier shipping.

Is lyophilised CEE sterile?

The material is uncapped during lyophilisation, so sterility can’t be assured. Filter lyophilised product through a 0.2 µm filter after reconstitution. If required, add extra antibiotic/antimycotic after filtering.

Can I test a batch before I buy?

Yes. We can send a 10 mL sample, and we hold a reserved batch for four weeks while you evaluate it.

Can I get CEE without antibiotics?

Yes. CEE is available without antibiotics, as an ultrafiltrate, and in custom sizes.

How should CEE be stored and shipped?

Store frozen CEE at -20 °C or below; lyophilised CEE can be stored at +4 °C. Frozen product ships on dry ice and lyophilised product on blue ice. Shelf life is 5 years from manufacture.

How do I reconstitute lyophilised CEE?

Reconstitute to the original volume of 10 mL with sterile tissue culture grade water and agitate to mix. If a precipitate appears, keep agitating under cool conditions for several hours or overnight, or sonicate briefly in a sonicating water bath. Centrifuge at 3,000 g for 10 minutes and remove the pellet. Once reconstituted, store at 4 °C for immediate use, or aliquot into working volumes and freeze.

What should I do if I see a precipitate after thawing frozen CEE?

Protein precipitate may be seen on thawing. Centrifuge at 3,000 g for 10 minutes to remove it. To avoid repeated freeze–thaw cycles, aliquot frozen CEE into working volumes before long-term storage.

Downloads

Download the Chick Embryo Extract Product Information Sheet (PDF)